Journal: bioRxiv

Article Title: A third type of PETase from the marine Halopseudomonas lineage

doi: 10.1101/2024.12.31.630877

Figure Lengend Snippet: a) Loops of interest in crystal structures of LCC (PDB: 4eb0), Haes _PE-H (PDB: 6sbn), Is PETase (PDB: 5xjh) and Halo PETase1 (PDB: 9hl5) are colored green. These loops are numbered for Halo PETase1. b) Corresponding sequence alignments for loop regions in a . c) Aligned ColabFold model structures of low bit-score hits from Halopseudomonas proteome including Halo PETase2, 3, and 4. Structure alignment was performed globally with align in PyMOL over backbone C α atoms (5 refinement cycles, >2 Å outlier rejection) without the first 40 residues to exclude the N-terminal signal peptides. Corresponding loops are highlighted in red (loop 1), orange (loop 2), and yellow (loop 3). d) Logo plot of aligned loop regions for low bit-score hits.

Article Snippet: All structural representations and graphical manipulations were performed in PyMOL 3.0.0 (Schrodinger, LLC) .

Techniques: Sequencing